Mammalian cells are currently the primary system for the production of recombinant proteins for clinical applications due to their capability for proper protein folding, assembly and post-translational modification. These mechanisms ensure the quality and efficacy of protein of interest being produced compared to bacterial systems.
Recently, the productivity in the range of more than 1 gram per liter has been achieved in mammalian cells cultivated in bioreactors as a result of improvements in media formulation, clonal selection and cell line development. Cell line development services offered by the Protein Science team at Inno Biologics cover molecular biology, cell culture, protein expression and verification.
CLONING AND VERIFICATION OF GENE EXPRESSION
Isolation of gene of interest from biological sources.Cloning of cDNA encoding protein of interest.Purification of plasmids/vectors.Gene expression analysis.
Transfection of expression vectors into mammalian cells.Screening of transfected cells.
Verification of protein expression and identity.Expansion of selected clones.
ESTABLISHMENT OF CELL LINES
Selection of high-producing clones using ClonePixTM FL system.Determination of expression level of selected clones.Establishment of R&D working and master cell banks.
Various bioassays and analytical tools – PCR, ELISA, SDS-PAGE, etc.